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A transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters
The first generation transgenic crops used strong constitutive promoters for transgene expression.
However, tissue-specific expression is desirable for more precise targeting of transgenes. Moreover,
piercing/sucking ...
RNA Interference based Approach to Down Regulate Osmoregulators of Whitefly (Bemisia tabaci): Potential Technology for the Control of Whitefly
Over the past decade RNA interference (RNAi) technology has emerged as a successful tool not only for functional genomics, but in planta expression of short interfering RNAs (siRNAs) that could offer great potential for ...
Recombination Among Begomoviruses on Malvaceous PlantsLeads to the Evolution of Okra Enation Leaf Curl Virus in Pakistan
Whitefly transmitted begomoviruses (family Geminiviridae) are the majorreason for significant yield losses of dicotyledonous crops in tropics andsubtropics. Okra (Abelmoschus esculentus) is one of the important vegetablecrops, ...
Regional changes in the sequence of cotton leaf curl multan betasatellite
Cotton leaf curl disease (CLCuD) in Pakistan and northwestern India is caused by monopartite begomoviruses in association with an essential, disease-specific satellite, Cotton leaf curl Multan betasatellite (CLCuMB). ...
Xanthium strumarium: a weed host of components of begomovirus-betasatellite complexes affecting crops
Xanthium strumarium is a common weed that often shows symptoms typical of begomovirus infection, such as leaf curling and vein thickening. The virus complex isolated from the weed consisted of two begomoviruses along with ...
Nutrients Can Enhance the Abundance and Expression of Alkane Hydroxylase CYP153 Gene in the Rhizosphere of Ryegrass Planted in Hydrocarbon-Polluted Soil
Plant-bacteria partnership is a promising strategy for the remediation of soil and water polluted with hydrocarbons. However, the limitation of major nutrients (N, P and K) in soil affects the survival and metabolic activity ...
Artificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virus
Background: Cotton leaf curl disease, caused by single-stranded DNA viruses of the genus Begomovirus (family Geminiviridae), is a major constraint to cotton cultivation across Pakistan and north-western India. At this time ...
Amplicon-based RNA interference targeting V2 gene of cotton leaf curl kokhran virus- burewala strain can provide resistance in transgenic cotton plants
The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation ...
First Report of Chilli leaf curl virus and Associated Alpha- and Beta-satellite DNAs Infecting Nettle Weed (Urtica dioica) in Pakistan
Weeds act as reservoirs of begomoviruses and ‘‘mixing vessels’’ leading to emergence of new viruses that may infect crops once a susceptible host plant is introduced into an agro-ecological system. Owing to the importance ...
Virus-Induced Gene Silencing in Cultivated Cotton (Gossypium spp.) Using Tobacco Rattle Virus.
The study described here has optimized the conditions for virus-induced gene silencing (VIGS) in three cultivated cotton species (Gossypium hirsutum, G. arboreum, and G. herbaceum) using a Tobacco rattle virus (TRV) vector. ...