Genotyping of 10000 individuals annually to make early generation selection decisions

Views
0% 0
Downloads
0 0%

Timeless limited access

Thumbnail Image

View/Open

Corresponding Author

Date

2019-07-01

Date Issued

Authors

Citation

Pasupuleti Janila, Sunil Chaudhari. (1/7/2019). Genotyping of 10000 individuals annually to make early generation selection decisions.

Abstract

Genotyping for FAD mutant alleles was initially done using allele-specific (AS-PCR) and cleaved amplified polymorphic sequence (CAPS) markers for detection of heterozygotes and homozygotes, respectively (Janila et al., 2016). This enabled better resource utilization but was cost intensive genotyping of one sample using CAPS marker costs 3.0 USD. The protocol for DNA isolation using seed chip is also standardized which will be helpful to minimize the resource required for genotyping (Figure 3). It reduces the time and resources required for planting and labeling individual plants in the field.

Permanent link

https://hdl.handle.net/20.500.11766/10346

DOI

Collections

Agricultural Research Knowledge
Agriculture and Livelihood systems

Other URI

https://mel.cgiar.org/dspace/limited

AGROVOC Keyword(s)

genotyping

Subject(s)

selection decision in early generations; cost-effective genotyping using snps

Author(s) ORCID(s)

Janila, Pasupuleti  https://orcid.org/0000-0003-2583-9630
 Chaudhari, Sunil  https://orcid.org/0000-0003-1441-3327