In vitro screening for low methane forages and their in vivo evaluation: Approaches and pathways


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Alejandra Marin, Harinder P. S. Makkar, Muhi El-Dine Hilali, Bayissa Hatew, Jane Wamatu, Juan Gonzalez, Yonas Asmare, Peter Wenzl, Isabel Molina, Johanna Mazabel, Stefan Muetzel, Andres Botero, Carolina Clavijo, Jacobo Arango. (5/10/2025). In vitro screening for low methane forages and their in vivo evaluation: Approaches and pathways. Colombia: Alliance Bioversity International-International Center for Tropical Agriculture (ABC).
CH₄ emissions from ruminants are a major source of agricultural GHGs. CGIAR genebanks (Alliance, ILRI, and ICARDA) hold rich genetic diversity to discover low-methane forages (LMFs). A funnel-shaped screening approach was developed to progressively narrow down thousands of accessions to the most promising candidates for methane mitigation and high digestibility.

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